Select those primers with one band in the gel picture and one peak in the dissociation curve for further experiments. If you have not yet used them we recommend trying our new ssoadvanced universal sybr green and ssoadvanced universal probes supermixes as superior performance alternatives for use on any real time pcr instrument. Quantitative rt pcr protocol sybr green i 5 3 running 3 agarose gel after qrt pcr 4 analyze qrt pcr data.
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