Sybr Green Qpcr Mix

Lna enhanced primer sets require specific pcr reaction conditions for optimal performance.

Sybr green qpcr mix. Applied biosystems power sybr green pcr master mix delivers highly sensitive nucleic acid quantitation detecting as few as 2 copies of a target gene over a broad range of template concentrations. Bimake 2x sybr green qpcr master mix utilizes a special performance enhanced taq dna polymerase protected via a hot start activation technique and optimized qpcr buffer system to perform sybr green i based quantitative pcr. Simply add 25 μl of the 2 mix to dna template primers and water. In routine pcr the critical result is the final quantity of amplicon generated after the process.

Sybr green qpcr with the development of thermal cyclers incorporating fluorescent detection pcr has a new innovative application. Dye based quantitative pcr qpcr uses real time fluorescence of a double stranded dna dsdna binding dye most commonly sybr green i to measure dna amplification during each cycle of a pcr. The sybr green pcr master mix is a convenient premix of the components except primers template and water necessary to perform real time pcr using sybr green i dye. Direct detection of pcr product is monitored by measuring the increase in fluorescence caused by the binding of sybr green dye to double stranded ds dna.

The detection is based on sybr green which allows quality control of the resulting pcr amplicon by melting curve analysis. The master mixes contain buffer dntps thermostable hot start dna polymerase and of course sybr green dye everything needed for real time pcr except the sample and pcr primer pair. The newly optimized formulation contains highly purified applied biosystems amplitaq gold dna polymerase ld to offer greater sensitivity. Sybr green master mixes are 2x concentrated and contain dntps mgcl 2 and dna polymerase.

Sybr green master mixes are available with other dyes including rox. This ready to use mixture of sybr green i jumpstart taq dna polymerase 99 pure deoxynucleotides and reaction buffer is provided in a 2 concentrate for ease of use. An internal reference dye such as rox corrects well to well optical variations and is used for fluorescent signal normalization. The mix prepared at 2x reaction concentration can be directly used for robust and low template quantitative pcr with.

Many pcr machines require a passive reference dye. Itaq universal sybr green supermix is a 2x concentrated ready to use reaction master mix optimized for dye based quantitative pcr qpcr on any real time pcr instrument.